Adalimumab ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of Adaliumab with high sensitivity and specificity in serum and plasma.
Kit Performance
Sensitivity: 25 ng/mL
Assay Range: 25 – 2000 ng/mL
Cross-reactivity: There is no cross reaction with native serum immunoglobulins
Recovery rate: 80 – 120% with normal human serum samples with known concentrations.
Precision
(CV (%) = SD/mean X 100)
Intra-Assay: CV < 20%
Inter-Assay: CV < 20%
Materials Supplied
List of component
Components
Quantity
High binding Plate
1 plate
Adaliumab
10 µg
10x Capture
700 µL
10x Detection
700 µL
1x Streptavidin (HRP) (Avoid light)
12 mL
1x Plate Coating Buffer
15 mL
1x Blocking Buffer
15 mL
2x Assay Diluent
30 mL
1x TMB Substrate (Avoid light)
12 mL
1x Stop Solution
12 mL
Storage Instruction
The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.
