C-peptide ELISA Kit

C-peptide ELISA Kit 

Catalog Number: CPEP-01-1156

Size: 1 plate (96 wells)

C-peptide ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of C-peptide with high sensitivity and specificity in serum or plasma.

Kit Performance

• Sensitivity: 1 pmol/L
• Assay Range: 1 – 500 pmol/L
• Cross-reactivity: There is no cross reaction with native immunoglobulins
• Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
• Precision

(CV (%) = SD/mean X 100)

• Intra-Assay: CV < 20%
• Inter-Assay: CV < 20%

Materials Supplied

List of component

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

Supplementary information

C-peptide (Connecting peptide) is a short peptide released during the production of insulin in pancreatic β-cells. Insulin is initially synthesized as preproinsulin, which is then processed into proinsulin in the endoplasmic reticulum. During maturation in secretory granules, enzymes cleave proinsulin to form active insulin (A chain + B chain) and the C-peptide, which connects these two chains in the precursor molecule.

Clinically, C-peptide measurement is commonly used to evaluate pancreatic β-cell function. Low or absent C-peptide levels suggest impaired insulin production, as seen in type 1 diabetes or advanced type 2 diabetes, while elevated levels may occur in conditions with excessive insulin secretion, such as insulinoma or insulin resistance.