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4-Hydroxynonenal ELISA Kit

4-Hydroxynonenal ELISA Kit
4-Hydroxynonenal ELISA Kit
  • Stock: In Stock
$850.00

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Description

4-Hydroxynonenal ELISA Kit 

Catalog Number: HNE4-01-1053

Size: 1 plate (96 wells)

4-Hydroxynonenal ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of 4-Hydroxynonenal with high sensitivity and specificity in serum or plasma.

   

Kit Performance

  • Sensitivity: 0.1 ng/mL
  • Assay Range: 0.1 – 20 ng/mL
  • Cross-reactivity: There is no cross reaction with native immunoglobulins
  • Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
  • Precision

(CV (%) = SD/mean X 100)

  • Intra-Assay: CV < 20%
  • Inter-Assay: CV < 20%

Materials Supplied

List of component

Components

  Quantity

High binding Plate

  1 plate

4-Hydroxynonenal

  1 µg

10x Capture

  700 µL

10x Detection

  700 µL

1x Streptavidin (HRP) (Avoid light)

  12 mL

1x Plate Coating Buffer

  15 mL

1x Blocking Buffer

  15 mL

2x Assay Diluent

  30 mL

1x TMB Substrate (Avoid light)

  12 mL

1x Stop Solution

  12 mL

 

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

 

Supplementary information

4-Hydroxynonenal (4-HNE) is a reactive aldehyde produced during lipid peroxidation, a process that occurs when reactive oxygen species (ROS) damage cell membrane lipids. Because it forms when lipids are oxidized, 4-HNE is widely used as a biomarker of oxidative stress.

Elevated levels of 4-HNE are associated with oxidative stress–related diseases, including neurodegenerative disorders, cardiovascular disease, metabolic diseases, and cancer.