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Description
4-Hydroxynonenal ELISA Kit
Catalog Number: HNE4-01-1053
Size: 1 plate (96 wells)
4-Hydroxynonenal ELISA Kit is a sandwich enzyme-linked
immunoassay for the quantitative determination of 4-Hydroxynonenal with high
sensitivity and specificity in serum or plasma.
Kit
Performance
- Sensitivity: 0.1 ng/mL
- Assay Range: 0.1 – 20 ng/mL
- Cross-reactivity: There is no
cross reaction with native immunoglobulins
- Recovery rate: 80 – 120% with
normal human serum or plasma samples with known concentrations.
- Precision
(CV
(%) = SD/mean X 100)
- Intra-Assay: CV < 20%
- Inter-Assay: CV < 20%
Materials
Supplied
List
of component
|
Components |
Quantity |
|
High binding Plate |
1 plate |
|
4-Hydroxynonenal |
1 µg |
|
10x Capture |
700 µL |
|
10x Detection |
700 µL |
|
1x Streptavidin (HRP) (Avoid
light) |
12 mL |
|
1x Plate Coating Buffer |
15 mL |
|
1x Blocking Buffer |
15 mL |
|
2x Assay Diluent |
30 mL |
|
1x TMB Substrate (Avoid light) |
12 mL |
|
1x Stop Solution |
12 mL |
Storage
Instruction
The
components arrive with ice pack, and must immediately be stored at 2-8°C. The
kit components are stable at the date specified in CoA.
Supplementary
information
4-Hydroxynonenal
(4-HNE) is a reactive aldehyde produced during lipid peroxidation, a process
that occurs when reactive oxygen species (ROS) damage cell membrane lipids.
Because it forms when lipids are oxidized, 4-HNE is widely used as a biomarker
of oxidative stress.
Elevated
levels of 4-HNE are associated with oxidative stress–related diseases,
including neurodegenerative disorders, cardiovascular disease, metabolic
diseases, and cancer.