VCAM-1 ELISA Kit

VCAM-1 ELISA Kit 

Catalog Number: VCAM1-01-1049

Size: 1 plate (96 wells)

VCAM-1 ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of VCAM-1 with high sensitivity and specificity in serum or plasma.

Kit Performance

• Sensitivity: 5 ng/mL
• Assay Range: 5 – 100 ng/mL
• Cross-reactivity: There is no cross reaction with native immunoglobulins
• Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
• Precision

(CV (%) = SD/mean X 100)

• Intra-Assay: CV < 20%
• Inter-Assay: CV < 20%

Materials Supplied

List of component

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

Supplementary information

VCAM-1 (Vascular Cell Adhesion Molecule-1) is a protein found on the surface of blood vessel cells (endothelial cells). Its main role is to help immune cells attach to blood vessels and move into tissues during inflammation.

Under normal conditions, VCAM-1 levels are very low. When the body experiences inflammation or infection, cytokines such as TNF-α and IL-1β stimulate endothelial cells to produce more VCAM-1.

VCAM-1 acts like a “molecular hook” on the blood vessel wall. It binds to specific proteins on immune cells, such as monocytes and lymphocytes, allowing these cells to stick to the vessel wall and migrate into inflamed tissue.

Because VCAM-1 increases during vascular inflammation, higher levels—especially soluble VCAM-1 in blood—are often associated with diseases such as atherosclerosis, cardiovascular disease, and diabetes. For this reason, VCAM-1 is commonly used as a biomarker of endothelial activation and vascular inflammation.