IL-10 ELISA Kit

IL-10 ELISA Kit 

Catalog Number: IL10-01-1041

Size: 1 plate (96 wells)

IL-10 ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of IL-10 with high sensitivity and specificity in serum or plasma.

Kit Performance

• Sensitivity: 1 pg/mL
• Assay Range: 1 – 100 pg/mL
• Cross-reactivity: There is no cross reaction with native immunoglobulins
• Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
• Precision

(CV (%) = SD/mean X 100)

• Intra-Assay: CV < 20%
• Inter-Assay: CV < 20%

Materials Supplied

List of component

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

Supplementary information

L-10 (Interleukin-10) is an important anti-inflammatory cytokine that plays a central role in regulating immune responses. Its primary function is to limit excessive inflammation and protect tissues from immune-mediated damage during infection or immune activation.

IL-10 is produced by several immune cell types, including regulatory T cells (Tregs), macrophages, monocytes, dendritic cells, and B cells. It is typically released during the later stages of an immune response, when the body needs to control or resolve inflammation after the initial immune activation.

IL-10 exerts its effects by binding to the IL-10 receptor (IL-10R) on target cells. This interaction activates intracellular signaling pathways, mainly the JAK–STAT pathway, particularly STAT3, which induces the expression of genes that suppress inflammatory responses. Biologically, IL-10 functions to inhibit the production of pro-inflammatory cytokines, including IL-1β, TNF-α, and IL-6, and reduces antigen presentation by macrophages and dendritic cells.