TNF alpha ELISA Kit

TNF alpha ELISA Kit 

Catalog Number: TNFA-01-1039

Size: 1 plate (96 wells)

TNF-α ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of TNF-α with high sensitivity and specificity in serum or plasma.

Kit Performance

• Sensitivity: 1 pg/mL
• Assay Range: 1 – 20 pg/mL
• Cross-reactivity: There is no cross reaction with native immunoglobulins
• Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
• Precision

(CV (%) = SD/mean X 100)

• Intra-Assay: CV < 20%
• Inter-Assay: CV < 20%

Materials Supplied

List of component

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

Supplementary information

TNF-α (Tumor Necrosis Factor-alpha) is a key pro-inflammatory cytokine that plays a central role in immune regulation and systemic inflammation. It is one of the earliest cytokines released in response to infection, tissue injury, or immune activation.

TNF-α is mainly produced by activated macrophages and monocytes, but it can also be released by T cells, natural killer (NK) cells, dendritic cells, and endothelial cells when the immune system detects pathogens or inflammatory signals.

TNF-α exerts its effects by binding to two receptors on target cells including TNFR1 (p55) and TNFR2 (p75). Activation of these receptors often triggers intracellular signaling pathways such as NF-κB and MAPK, leading to the expression of inflammatory genes.

During the inflammatory cascade, IL-1β and TNF-α are released early and stimulate the production of IL-6 and other cytokines. IL-1β primarily initiates the inflammatory response, whereas TNF-α amplifies inflammation and promotes broader immune activation.