IL-1β ELISA Kit

IL-1β ELISA Kit 

Catalog Number: IL1B -01-1037

Size: 1 plate (96 wells)

IL-1β ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of IL-1β with high sensitivity and specificity in serum or plasma.

Kit Performance

• Sensitivity: 5 pg/mL
• Assay Range: 5 – 100 pg/mL
• Cross-reactivity: There is no cross reaction with native immunoglobulins
• Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
• Precision

(CV (%) = SD/mean X 100)

• Intra-Assay: CV < 20%
• Inter-Assay: CV < 20%

Materials Supplied

List of component

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

Supplementary information

IL-1β (Interleukin-1 beta) is a potent pro-inflammatory cytokine that plays a central role in the initiation of inflammatory and immune responses.

IL-1β is mainly produced by monocytes, macrophages, and dendritic cells in response to infection, tissue damage, or inflammatory stimuli. It is first synthesized as an inactive precursor (pro-IL-1β). Activation occurs through the inflammasome complex, particularly the NLRP3 inflammasome, which activates caspase-1 to cleave pro-IL-1β into the active IL-1β protein.

Active IL-1β binds to the IL-1 receptor (IL-1R1) on target cells. This interaction triggers intracellular signaling pathways such as NF-κB and MAPK, which promote the expression of additional inflammatory mediators.