IgA ELISA Kit

IgA ELISA Kit 

Catalog Number: IGA-01-1159

Size: 1 plate (96 wells)

IgA ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of IgA with high sensitivity and specificity in serum or plasma.

Kit Performance

• Sensitivity: 0.2 ng/mL
• Assay Range: 0.2 – 50 ng/mL
• Cross-reactivity: There is no cross reaction with native immunoglobulins
• Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
• Precision

(CV (%) = SD/mean X 100)

• Intra-Assay: CV < 20%
• Inter-Assay: CV < 20%

Materials Supplied

List of component

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

Supplementary information

Immunoglobulin A (IgA) is an antibody class that plays a central role in mucosal immunity, protecting surfaces that are exposed to the external environment.

IgA exists in two main structural forms. In the bloodstream, IgA is usually present as a monomer, similar to IgG. In mucosal secretions, however, IgA typically forms a dimer, consisting of two antibody units linked by a J chain and associated with a secretory component that protects the antibody from enzymatic degradation. This secretory IgA structure allows it to function effectively in harsh environments such as the gastrointestinal tract.

The main function of IgA is immune exclusion, meaning it prevents pathogens and toxins from binding to and penetrating mucosal surfaces. Unlike some other antibodies, IgA generally does not strongly activate the complement system, which helps limit excessive inflammation in delicate mucosal tissues.

Clinically, abnormal IgA levels can be associated with immune disorders; for example, IgA deficiency is one of the most common primary immunodeficiencies and can increase susceptibility to infections of mucosal tissues.