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Description
LAMP-1 ELISA Kit
Catalog Number: LAMP1-01-1051
Size: 1 plate (96 wells)
LAMP-1 ELISA Kit is a sandwich enzyme-linked immunoassay for
the quantitative determination of LAMP-1 with high sensitivity and specificity
in serum or plasma.
Kit
Performance
- Sensitivity: 0.1 ng/mL
- Assay Range: 0.1 – 10 ng/mL
- Cross-reactivity: There is no
cross reaction with native immunoglobulins
- Recovery rate: 80 – 120% with
normal human serum or plasma samples with known concentrations.
- Precision
(CV
(%) = SD/mean X 100)
- Intra-Assay: CV < 20%
- Inter-Assay: CV < 20%
Materials
Supplied
List
of component
|
Components |
Quantity |
|
High binding Plate |
1 plate |
|
LAMP-1 |
1 µg |
|
10x Capture |
700 µL |
|
10x Detection |
700 µL |
|
1x Streptavidin (HRP) (Avoid
light) |
12 mL |
|
1x Plate Coating Buffer |
15 mL |
|
1x Blocking Buffer |
15 mL |
|
2x Assay Diluent |
30 mL |
|
1x TMB Substrate (Avoid light) |
12 mL |
|
1x Stop Solution |
12 mL |
Storage
Instruction
The
components arrive with ice pack, and must immediately be stored at 2-8°C. The
kit components are stable at the date specified in CoA.
Supplementary
information
LAMP-1
(Lysosome-Associated Membrane Protein-1) is a membrane glycoprotein mainly
located on lysosomes, which are intracellular organelles responsible for
protein degradation and cellular waste processing. LAMP-1 is one of the most
abundant proteins on the lysosomal membrane and helps maintain lysosome
structure and stability.
Under
normal conditions, LAMP-1 resides primarily on lysosomal membranes inside the
cell. However, during certain cellular processes such as lysosomal exocytosis
or immune cell activation, LAMP-1 can appear on the cell surface.
Because
lysosomal dysfunction is linked to several diseases, LAMP-1 is often studied in
conditions involving lysosomal storage disorders, neurodegenerative diseases,
and immune responses, and is commonly used as a biomarker of lysosomal activity
or immune cell activation in research.