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LAMP-1 ELISA Kit

LAMP-1 ELISA Kit
LAMP-1 ELISA Kit
  • Stock: In Stock
$850.00

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Description

LAMP-1 ELISA Kit 

Catalog Number: LAMP1-01-1051

Size: 1 plate (96 wells)

LAMP-1 ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of LAMP-1 with high sensitivity and specificity in serum or plasma.

  

Kit Performance

  • Sensitivity: 0.1 ng/mL
  • Assay Range: 0.1 – 10 ng/mL
  • Cross-reactivity: There is no cross reaction with native immunoglobulins
  • Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
  • Precision

(CV (%) = SD/mean X 100)

  • Intra-Assay: CV < 20%
  • Inter-Assay: CV < 20%

Materials Supplied

List of component

Components

  Quantity

High binding Plate

  1 plate

LAMP-1

  1 µg

10x Capture

  700 µL

10x Detection

  700 µL

1x Streptavidin (HRP) (Avoid light)

  12 mL

1x Plate Coating Buffer

  15 mL

1x Blocking Buffer

  15 mL

2x Assay Diluent

  30 mL

1x TMB Substrate (Avoid light)

  12 mL

1x Stop Solution

  12 mL

 

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

 

Supplementary information

LAMP-1 (Lysosome-Associated Membrane Protein-1) is a membrane glycoprotein mainly located on lysosomes, which are intracellular organelles responsible for protein degradation and cellular waste processing. LAMP-1 is one of the most abundant proteins on the lysosomal membrane and helps maintain lysosome structure and stability.

Under normal conditions, LAMP-1 resides primarily on lysosomal membranes inside the cell. However, during certain cellular processes such as lysosomal exocytosis or immune cell activation, LAMP-1 can appear on the cell surface.

Because lysosomal dysfunction is linked to several diseases, LAMP-1 is often studied in conditions involving lysosomal storage disorders, neurodegenerative diseases, and immune responses, and is commonly used as a biomarker of lysosomal activity or immune cell activation in research.