ICAM-1 ELISA Kit

ICAM-1 ELISA Kit 

Catalog Number: ICAM1-01-1050

Size: 1 plate (96 wells)

ICAM-1 ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of ICAM-1 with high sensitivity and specificity in serum or plasma.

Kit Performance

• Sensitivity: 1 ng/mL
• Assay Range: 1 – 50 ng/mL
• Cross-reactivity: There is no cross reaction with native immunoglobulins
• Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
• Precision

(CV (%) = SD/mean X 100)

• Intra-Assay: CV < 20%
• Inter-Assay: CV < 20%

Materials Supplied

List of component

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

Supplementary information

ICAM-1 (Intercellular Adhesion Molecule-1) is a cell surface protein expressed on endothelial cells and various immune cells. Its primary function is to help immune cells adhere to blood vessels and migrate into tissues during inflammation.

ICAM-1 mainly binds to the integrin LFA-1, which is expressed on many immune cells, including neutrophils, monocytes, and lymphocytes. In comparison, VCAM-1 primarily binds to VLA-4 (α4β1 integrin), which is found mainly on monocytes and lymphocytes. As a result, ICAM-1 supports the recruitment of a broader range of immune cells, whereas VCAM-1 tends to recruit more specific immune cell populations.

Biologically, ICAM-1 mainly facilitates general immune cell adhesion and migration during many inflammatory conditions, including infection and acute inflammation. In contrast, VCAM-1 is more closely associated with vascular inflammation and chronic diseases, particularly atherosclerosis, where it promotes monocyte recruitment into the vessel wall.