FGF21 ELISA Kit

FGF21 ELISA Kit 

Catalog Number: FGF21-01-1042

Size: 1 plate (96 wells)

FGF21 ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of FGF21 with high sensitivity and specificity in serum or plasma.

Kit Performance

• Sensitivity: 30 pg/mL
• Assay Range: 30 – 1000 pg/mL
• Cross-reactivity: There is no cross reaction with native immunoglobulins
• Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
• Precision

(CV (%) = SD/mean X 100)

• Intra-Assay: CV < 20%
• Inter-Assay: CV < 20%

Materials Supplied

List of component

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

Supplementary information

FGF21 (Fibroblast Growth Factor 21) is a metabolic hormone that plays an important role in regulating energy balance, glucose metabolism, and lipid metabolism.

FGF21 is mainly produced by the liver. Its production is stimulated by metabolic stress, such as fasting, ketogenic diets, cold exposure, and high-fat diets.

FGF21 exerts its effects by binding to FGF receptors (FGFRs) together with the co-receptor β-Klotho, which is highly expressed in metabolic tissues such as liver, adipose tissue, and the central nervous system. Activation of this receptor complex triggers intracellular signaling pathways, including MAPK/ERK signaling, leading to metabolic regulatory effects.

Biologically, FGF21 promotes glucose uptake, fatty acid oxidation, and energy expenditure, while also improving insulin sensitivity.