MCP-1 ELISA Kit

MCP-1 ELISA Kit 

Catalog Number: MCP-01-1040

Size: 1 plate (96 wells)

MCP-1 ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of MCP-1 with high sensitivity and specificity in serum or plasma.

Kit Performance

• Sensitivity: 30 pg/mL
• Assay Range: 30 – 1000 pg/mL
• Cross-reactivity: There is no cross reaction with native immunoglobulins
• Recovery rate: 80 – 120% with normal human serum or plasma samples with known concentrations.
• Precision

(CV (%) = SD/mean X 100)

• Intra-Assay: CV < 20%
• Inter-Assay: CV < 20%

Materials Supplied

List of component

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

Supplementary information

MCP-1 (Monocyte Chemoattractant Protein-1), also known as CCL2, is a chemokine that plays a key role in recruiting immune cells to sites of inflammation.

MCP-1 is produced by several cell types, including monocytes/macrophages, endothelial cells, fibroblasts, smooth muscle cells, and epithelial cells, in response to inflammatory stimuli such as IL-1β, TNF-α, and oxidative stress.

Once released, MCP-1 forms a concentration gradient around the site of inflammation. MCP-1 binds primarily to the CCR2 receptor on target cells, particularly monocytes, triggering intracellular signaling that promotes cell migration (chemotaxis).