α-Synuclein (phospho Ser129) (Human) ELISA Kit

α-Synuclein (phospho Ser129) (Human) ELISA Kit 

Catalog Number: ASYN- P129-01-1022

Size: 1 plate (96 wells)

α-Synuclein (pS129) ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of α-Synuclein (phospho Ser129) with high sensitivity and specificity in CSF.

Kit Performance

• Sensitivity: 0.05 ng/mL
• Assay Range: 0.05 – 2 ng/mL
• Cross-reactivity: There is no cross reaction found
• Recovery rate: 80 – 120% with normal human CSF samples with known concentrations.
• Precision

(CV (%) = SD/mean X 100)

• Intra-Assay: CV < 20%
• Inter-Assay: CV < 20%

Materials Supplied

List of component

Storage Instruction

The components arrive with ice pack, and must immediately be stored at 2-8°C. The kit components are stable at the date specified in CoA.

Supplementary information

α-Synuclein is a presynaptic neuronal protein involved in synaptic vesicle trafficking and neurotransmitter release. In Parkinson’s disease and related synucleinopathies, α-synuclein undergoes misfolding and aggregation, leading to the formation of Lewy bodies, a pathological hallmark of the disease.

Several α-synuclein species are commonly measured as biomarkers in research and clinical studies, including total α-synuclein, oligomeric α-synuclein, and phosphorylated α-synuclein. Total α-synuclein reflects the overall protein level, while oligomeric α-synuclein represents aggregated soluble forms that are considered the most neurotoxic species and are believed to play a key role in neuronal damage. In addition, phosphorylated α-synuclein at serine 129 is strongly associated with pathological aggregation, as the majority of α-synuclein present in Lewy bodies is phosphorylated at this residue.

Measurement of these different α-synuclein species helps characterize disease pathology, progression, and therapeutic response in Parkinson’s disease studies.